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SCIEN presents "High-speed 3D fluorescence microscopy with digital adaptive optics"

High-speed 3D fluorescence microscopy with digital adaptive optics
Wednesday, September 25, 2019 - 4:30pm
Packard 101
Dr. Jiamin Wu (Institute for Brain and Cognitive Sciences at Tsinghua University)
Abstract / Description: 

Observing large-scale three-dimensional subcellular dynamics in vivo at high spatiotemporal resolution has long been a pursuit for biology. However, both the signal-to-noise ratio and resolution degradation in multicellular organisms pose great challenges. In this talk, I will discuss our recent work in in vivo aberration-free 3D fluorescence imaging at millisecond scale by scanning light-field microscopy with digital adaptive optics. Specifically, we propose scanning light-field microscopy to achieve diffraction-limited 3D synthetic aperture for incoherent conditions, which facilitates real-time digital adaptive optics for every pixel in post-processing. Various fast subcellular processes are observed, including mitochondrial dynamics in cultured neurons, membrane dynamics in zebrafish embryos, and calcium propagations in cardiac cells, human cerebral organoids, and Drosophila larval neurons, enabling simultaneous in vivo studies of morphological and functional dynamics in 3D.


Jiamin Wu is a Postdoctoral Fellow within the Institute for Brain and Cognitive Sciences at Tsinghua University. His current research interests focus on computational microscopy and high-speed 3D imaging, with a particular emphasis on developing computation-based optical setups for observing large-scale biological dynamics in vivo. He received his PhD degree (2019) and bachelor's degree (2014) in the Department of Automation from Tsinghua University under the supervisor of Professor Qionghai Dai.