Proteins and nucleic acids are dynamically organized in cells to realize their physiological functions with spatial and temporal orderliness. This type of elegant supermolecular assembly has inspired researchers to create molecular/biomolecular structures with dynamic organization outside of the cells. In particular, DNA nanotechnology has proven to possess extraordinary flexibility and convenience for "bottom-up" construction of exquisite nanostructures with high controllability and precision, which holds great promise in a wide range of applications, e.g., nanofabrication and molecular electronics, in-vivo and in-vitro sensing and drug delivery.
In this talk, I will present several examples of using tetrahedral DNA nanostructures (TDNs) for engineering the interfaces of cytoplasmic membranes and biosensors. TDNs are three-dimensional (3D) DNA architecture with high mechanical rigidity and structural stability, which are suitable for organization of higher-ordered nanocomplexes and nanodevices. In one example, we employed single-particle tracking to visualize the internalization of TDNs, and dissect the cell entry pathways of these virus-like nanoparticles. In the second example, we dynamically organized biomolecular receptors at the biosensing interface using TDNs, and performed in-vitro diagnostics for various diseases.
Chunhai Fan obtained his B.S. and Ph.D. from the Department of Biochemistry at Nanjing University in 1996 and 2000. After his postdoctoral research at University of California, Santa Barbara (UCSB), he joined the faculty at Shanghai Institute of Applied Physics (SINAP), Chinese Academy of Sciences (CAS) in 2004. He is now Professor and Chief of the Division of Physical Biology at SINAP and the Bioimaging Center of Shanghai Synchrotron Radiation Facility (SSRF). He is a fellow of Royal Society of Chemistry (FRSC) and an elected fellow of the International Society of Electrochemistry (ISE). He is also an Associate Editor of ACS Applied Materials & Interfaces, a Guest Editor of Advanced Materials, and serves on the editorial board of several international journals (ChemBioChem, ChemPlusChem, Scientific Reports, ACS Sensors, Advanced Healthcare Materials, Journal of Materials Chemistry, Electroanalysis, Particles etc.). Dr. Fan has published ~300 papers in peer-reviewed journals, and he was recently recognized as High Cited Researchers by Thomson Reuters.