H. Tom Soh

Wednesday, October 9, 2013 (All day)

Packard

Abstract

"Cell Sorting and Directed Evolution in Microfluidic Systems"

Current techniques in high performance molecular and cellular separations are limited by the inherent coupling among three competing parameters: throughput, purity, and recovery of rare species. Our group utilizes unique advantages of microfluidics technology to decouple these competing parameters by precise and reproducible generation of separation forces that are not accessible in conventional, macroscopic systems. In this seminar, we will first discuss novel high performance electrokinetic, magnetophoretic and acoustophoretic separation systems to purify rare target cells from complex mixtures. Next, we will discuss our recent work in applying the microfluidic separation systems for Rapid Directed Evolution of molecules (RDE). We will provide theoretical and experimental evidence for extremely fast generation of affinity reagents –molecular recognition elements that bind to target molecules with high affinity and specificity. Finally, we will present innovative methods of evolving molecular machines that are capable of performing complex functions, including binding induced conformation change and switching.

Biography

Dr. Soh received his B.S. with a double major in Mechanical Engineering and Materials Science with Distinction from Cornell University, and Ph.D. in Electrical Engineering from Stanford University. Prior to joining UCSB in 2003, Dr. Soh served as the technical manager of MEMS Device Research Group at Bell Laboratories and MEMS R&D group at Agere Systems. His current research interests are in analytical biotechnology, especially in high throughput screening, directed evolution and integrated biosensors. He is Co-Director at the Center for Stem Cell Biology & Engineering and Associate Director of the California Nanosystems Institute (CNSI). He is the recipient of MIT Technology Review’s "TR 100" Award (2002), ONR Young Investigator Award (2004), Beckman Young Investigator Award (2005), ALA Innovator Award (2009), NIH Director’s TR01 Award (2009), John Simon Guggenheim Fellowship (2010), NIH Edward Nagy Award (2011), Garland Endowed Chairship at UCSB (2011), and Humboldt Research Fellowship (2012).

Video

H. Tom Soh

Wednesday, October 9, 2013 (All day)
Packard
Abstract

Current techniques in high performance molecular and cellular separations are limited by the inherent coupling among three competing parameters: throughput, purity, and recovery of rare species. Our group utilizes unique advantages of microfluidics technology to decouple these competing parameters by precise and reproducible generation of separation forces that are not accessible in conventional, macroscopic systems. In this seminar, we will first discuss novel high performance electrokinetic, magnetophoretic and acoustophoretic separation systems to purify rare target cells from complex mixtures. Next, we will discuss our recent work in applying the microfluidic separation systems for Rapid Directed Evolution of molecules (RDE). We will provide theoretical and experimental evidence for extremely fast generation of affinity reagents –molecular recognition elements that bind to target molecules with high affinity and specificity. Finally, we will present innovative methods of evolving molecular machines that are capable of performing complex functions, including binding induced conformation change and switching.